Re: [Histonet] Leica RM2255 holder for round specimens

Hi Teri,
        I suggest you use the "Standard Specimen Clamp with  the "V" notch 
insert. Plastic embedded specimens are hard enough to clamp directly 
without crushing. (14050237998 and 14050238000 ). If you have further 
questions, please call.
Best regards,

Don Birgerson
Leica Microsystems
Technical Assistance Center 
1-800-248-0123  ext 5918

"Johnson, Teri"  
Sent by:
08/25/2005 08:34 AM


[Histonet] Leica RM2255 holder for round specimens

I just bought the accessory for our RM2255 microtome so we could use it
for sectioning our PMMA embedded samples.  The inserts that came with it
are 6, 15, and 25 mm in diameter.  It appears that unless the diameter
of the block is almost exactly this measurement, the holder will not
work.  Is this truly the case or is there some adjustment we aren't
aware of?  Unfortunately as well, the vials we used for embedding are
smaller than 25 mm but larger than 15.  We plan to either mount them
onto a block holder with adhesive and try to cut them that way, or use a
dremel or other power tool to reduce the diameter TO 15 mm.  Are there
other things we can do that I'm just not seeing?


Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

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