Steve, 
What pretreatment are you using? Is your detection system avidin-biotin
based or a polymer? What is the concentration of primary antibody? Does your
negative exhibit any of this background staining?
Sorry for all the questions, but I can help more once I know the answers.
This is not a problem we've seen with the antibody we use.

Patti Loykasek, BS,HTL, QIHC
PhenoPath Laboratories
Seattle, WA

 
I have been getting a lot of background in the mucosa of the Gastric
> biopsies, especially in cases that show increased inflammation and many
> eosinophils. I 
> have increased the incubation time in peroxide blocker and protein blocker
> and decreased the time in the primary and secondary Ab, only to find it
> getting 
> worse instead of better. I am using Biocare's antibody and detection system on
> their instrument. Any suggestions to eliminate this nonspecific staining?
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