Re: [Histonet] Cutting defect and blade adjustement

From:mucram11@comcast.net

Hi Jorge,

Could you please give us your processing protocol?  It would help to know if the schedule is too long and you over dehydrating if could cause you problems with very hard tissue components.  If the blocks are not fully glued down and any wiggle at all ocurrs this could also be a problem.  The product you are using contains GMA I believe and it is very water sensitive and will gather water from the  just the enviroment so you may wish to stoe them in a chamber with a a dissicant to assure they are completely polymerized and free of excess water.  Hope this helps.

Pam Marcum
UPenn Vet Schooll
-------------- Original message -------------- 

> My blocks are 12 mm wide by 16 mm long, and they are about 5 mm thick. 
> They are glued (with technovit 3090) to a small piece of wood and this 
> is the piece that I hold with the specimen holder. I am sectioning 
> anchovy ovaries, and I am using a non standard protocol for the 
> embedding, with much longer processing times, I guess because of the 
> very high water content of the tissue. I wonder if it is better to 
> make more changes in the alcohols instead of long times. 
> 
> I am sorry but no way for using glass knives or diamond (budgets) 
> 
> Thank you very much for your responses, I am waiting for more if you 
> want, any help would be appreciated 
> 
> Jorge Tornero 
> IEO-CADIZ 
> Spain 
> 
> 2005/8/30, meyenhmf@umdnj.edu : 
> > How big are your blocks? Thickness?Tissue? 
> > I would use glass knives or Diamond. 
> > Metal blades always give streaks. 
> > Regards, 
> > Markus 
> > 
> > 
> > 
> > forQuoting Jorge Tornero : 
> > 
> > > Please help me about the right knife angle for cutting technovit 7100 
> > > blocks with a leica tungsten carbide knife. I use a leica RM 2145 
> > > microtome. 
> > > 
> > > I am using a freshly sharpened knife, but I get some sharp lines on 
> > > the cuttings (you know, those that appear all the way long in the cut 
> > > and in the same place) anyway. It is the first time that the knife is 
> > > sharpened. Also I hear a buzzing noise when I cut, it seems like if 
> > > the block causes some vibration in the blade, but it is well fastened. 
> > > I have tried to decrease speed when cutting but it doesn't work at 
> > > all. Now I am using a kind of moulds bigger than those that I used 
> > > before, I don't know if I should trim the block edge to make it 
> > > narrower and ease the cutting this way. any suggestions, please? 
> > > 
> > > Thank you very much 
> > > 
> > > Jorge Tornero 
> > > IEO-CADIZ 
> > > Spain 
> > > 
> > > _______________________________________________ 
> > > Histonet mailing list 
> > > Histonet@lists.utsouthwestern.edu 
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> > > 
> > 
> > 
> > -- 
> > 
> > 
> 
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