Re: [Histonet] Breast Processing Protocol
Douglas and fellow histonetters,
The major problem with processing fatty tissues, assuming that they are
fixed properly, is that Ethanol is not a good solvent for fat and therefore
connot penetrate the tissues completely, so the tissues are inadequately
We have had some success with lipomas by adding a "degreasing" step of
xylene into the processing schedule.
The first Absolute ethanol will dehydrate the tissue to some extent. The
next Xylene step will remove most of the fat and then the second Absolute
ethanol can complete the dehydration.
A compromise situation that we use routinely is to have
50:50 Absolute ethanol:Xylene
This is not quite as effective but it is less disruptive to the normal
schedule and handles moderately fatty tissues.
Regards from Townsville, Australia.
At 09:58 AM 17/08/2005 -0400, DDDeltour@mar.med.navy.mil wrote:
>I am looking for a program for processing fatty breast tissue. We currently
>let the breast sit in cassettes in Pen-Fix for an extra day before
>processing. We then process the first Fixative in Pen-Fix for one hour then
>in 10% Formalin for the second hour (I do not like this but that is they way
>it was done when I got here). The next steps are.. 95% one hour X3, 100% one
>hour X3, Xylene 1 hour X2. Paraffin for one hour X3. I am considering
>replacing one of the 95% with another fixative. What programs do you use for
>this? They don't like to hear Sh in Sh out:-). Thank you!
>DOUGLAS D. DELTOUR
>NMC PORTSMOUTH VA
>Histonet mailing list
Mr.Laurie Reilly Ph 07 4781 4468
School of Veterinary & Biomedical Science Fax 07 4779 1526
James Cook University
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