Re: [Histonet] Ahh, my trichromes are green again

From:Geoff McAuliffe

This is a good argument for making your own solutions. That way you will 
know exactly what is in them and what the pH is.

Geoff

Hofecker, Jennifer L wrote:

>Hi everyone,
>Just wanted to thank the many people who gave me helpful hints.  We found that our commercially prepared Gomori trichrome solution was at pH 2.0!  We brought it up to 3.4 and it worked great.  We have ordered new solution and I plan on checking the pH when it comes in.  We also decided to wait a few minutes after cutting frozen sections.  Several people recommended both of these things and we are very grateful.
>Have a great rest of the week.
> 
>Jennifer
> 
> 
> 
>Jennifer Hofecker, HT (ASCP)
>Vanderbilt University Medical Center
>Division of Neuropathology
>(615) 343-0083
>(615) 343-7089 fax
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>  
>


-- 
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
**********************************************



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