Liz, various enzymes break peptides at different sites. Maybe the authors
found that chymotrypsin works better for cartlilage.

See info at:
http://www.greatvistachemicals.com/biochemicals/chymotrypsin.html

Tim Morken
Lab Vision - Neomarkers
www.labvision.com

Free webhosting for US State Histotechnology Societies:
http://www.labvisioncorp.com/demowebsite/index.cfm




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Monday, August 29, 2005 1:38 PM
To: 'Histonet'
Subject: [Histonet] trypsin verses chymotrypsin


Hello everyone
 
Is there anyone out there that can explain the difference between trypsin
and chymotrypsin for retrieval in immunohistochemistry.  I'm working out a
protocol for aggrecan on cartilage and I have a protocol that says they
retrieved in chymotrypsin.  Any input would be appreciated.
 
Thanks in advance
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 
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