RE: [Histonet] trypsin verses chymotrypsin

From:"Morken, Tim - Labvision"

Liz, various enzymes break peptides at different sites. Maybe the authors
found that chymotrypsin works better for cartlilage.

See info at:

Tim Morken
Lab Vision - Neomarkers

Free webhosting for US State Histotechnology Societies:

-----Original Message-----
[] On Behalf Of Elizabeth
Sent: Monday, August 29, 2005 1:38 PM
To: 'Histonet'
Subject: [Histonet] trypsin verses chymotrypsin

Hello everyone
Is there anyone out there that can explain the difference between trypsin
and chymotrypsin for retrieval in immunohistochemistry.  I'm working out a
protocol for aggrecan on cartilage and I have a protocol that says they
retrieved in chymotrypsin.  Any input would be appreciated.
Thanks in advance
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
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