RE: [Histonet] trypsin verses chymotrypsin

From:"Goodpaster, Tracy A"

I would be interested in any comments on this topic as well!


From: on behalf of Elizabeth Chlipala
Sent: Mon 8/29/2005 1:37 PM
To: 'Histonet'
Subject: [Histonet] trypsin verses chymotrypsin

Hello everyone

Is there anyone out there that can explain the difference between
trypsin and chymotrypsin for retrieval in immunohistochemistry.  I'm
working out a protocol for aggrecan on cartilage and I have a protocol
that says they retrieved in chymotrypsin.  Any input would be

Thanks in advance


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540

Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

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