RE: [Histonet] rat heart cryos' for immunos

From:"Pereira, Laurie "

We are using isopentane and it takes about a minute to freeze the tissue.  There appears to be cracks between the muscle fibers.

-----Original Message-----
From: Kristen Broomall [mailto:kbroomal@NEMOURS.ORG]
Sent: Friday, August 12, 2005 10:24 AM
To: Pereira, Laurie ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] rat heart cryos' for immunos


How are you freezing your tissue? It's probably freezing artifact. Does it
look like big donut holes in the middle of the muscle fibers?

Kristen Broomall

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pereira,
Laurie 
Sent: Friday, August 12, 2005 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] rat heart cryos' for immunos



Does anyone know why heart cryos tend to separate?  We've tried fresh
frozen, NBF perfused, sucrose perfused and FBS perfused and the heart tissue
has 'holes' in it.
Laurie L. Pereira, RVT
SDCADDL (County Veterinarian)
5555 Overland Avenue Suite 4103
San Diego, CA. 92123
Phone: 858-694-3384
Fax: 858-571-4268


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