RE: [Histonet] puzzling morphology

From:"Rogerson Kemlo (ELHT) Pathology"

It's not actually an absence of tissue is it? The spaces could be due to
virus, like koilocytotic atypia. It could also be an artefact as you
suggest but I can't figure what could cause perinuclear haloing as you
suggest, I would have thought cellular changes would have affected the
whole cell not just shrinking the nucleus. 

-----Original Message-----
[] On Behalf Of Walters,
Katherine S
Sent: 23 August 2005 22:53
Subject: [Histonet] puzzling morphology

I am once again in need of some collective wisdom from the experts.

I have some paraffin embedded lung tissue in which the bronchial
endothelium cells exhibit "spaces" (around the nucleus) in the cytoplasm
in the H&E 5 micron section.  These were fixed with zinc formalin.

Does anyone know what would cause this absence of tissue?

Thanks again for your wisdom,

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