RE: [Histonet] (no subject)

From:"Patsy Ruegg"

We used picro sirrus stain for collagen fibers and viewed the collagen
bundles using polarized light filter for histomorphometry.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
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-----Original Message-----
[] On Behalf Of
Sent: Sunday, July 31, 2005 3:18 AM
Subject: [Histonet] (no subject)

Dear Subscribers

I am staining cartilage using Millers elastic fibre stain, and while elastic
fibres are staining well, there is a lot of blue background staining which
makes thresholding for histoquantitation almost impossible. My sections are
30 micron paraffin embedded. I am also oxidising with 0.5% aqueous potassium
permanaganate and bleaching with 2% oxalic acid.

Any suggestings for reducing this background staining would be greatly

Kind regards,

Lachlan Smith
Institute of Medical and Veterinary Science Adelaide, Australia

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