The smears can either be air-dried or ethanol fixed. I have found better
cellular retention with air-drying. 
After fixation (see above) do a PAS, or a mucicarmine or the Alcian
Blue-Argentaffin reaction for the cryptococcus.

For other fungi, a PAS or Chromic acid-Methenamine Silver or a Sulphated
Toluidine Blue (good for pneumocystis)can be done.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
RStaskiewicz@agr.state.il.us
Sent: Thursday, 25 August 2005 1:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Stain for fungi in cytological smear


Good morning all! I have a request to stain a cytological smear of a nasal 
wash for cryptococcus. I have never stained smears for fungi, just tissue. 
Does anyone have any experience or suggestions?


Rae Ann Staskiewicz HT(ASCP)
Section Head-Histology
Galesburg Animal Disease Lab
2100 S. Lake Storey Rd
Galesburg, IL 61401
(309) 344-2451
rstaskiewicz@agr.state.il.us _______________________________________________
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