RE: [Histonet] Re: ooops, rat on rat

From:"Mandy Townsend"

Serotec are able to supply a polymer based kit that is suitable for use in rat on rat (and mouse on mouse) protocols - STAR4000A.  Please contact our US office for further information.

Serotec North & South America (website)
3200 Atlantic Avenue, Suite 105
Raleigh, NC 27604, USA
Toll free: 1-800-265-7376 
Fax: 919-878-3751 
Product enquiry

I hope this helps.

Mandy Townsend MSc
Technical Services Advisor
Serotec Ltd
22 Bankside
Station Approach
OX5 1JE 

Tel: +44 1865 852733
Fax: +44 1865 852739
Serotec-Your first choice for antibodies! 

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-----Original Message-----
From: []On Behalf Of Gayle Callis
Sent: Tuesday, August 02, 2005 9:21 PM
To: Till, Renee;
Subject: [Histonet] Re: ooops, rat on rat

You could use a biotinylated primary, as in the DAKO ARK methodology,
eliminate the secondary to avoid having some "host" antiRat detect not only
the rat primary, but also bind to rat tissue, then come back with a
Strepavidin-HRP or AP.  Sometimes you have to dilute out the primary more -

I am not sure if anyone has a rat on rat kit, try Scytek out of Logan UT,
you might get lucky on a kit.

At 01:49 PM 8/2/2005, you wrote:
>Okay, I knew I'd say that wrong. It is a rat anti-mouse antibody to be
>used on rat tissues. How would you handle that?
>Renee' Till, HT
>Arkansas Childrens Nutrition Center
>1120 Marshall
>Little Rock, AR
>(501) 364-2774
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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