RE: [Histonet] Orcein for copper-binding protein/Hep B

From:"Lee & Peggy Wenk"

Are you looking for copper or for the hepatitis B?

If I remember correctly, orcein stains the hepatitis B inclusion body (the
ground glass appearance on an H&E)and it also stains elastin. Other stains
for the hepatitis B were the aldehyde fuchsin and the Victoria blue. I think
most labs do an IHC for hepatitis, as you can differentiate Hepatitis B core
from Hepatitis B surface antigens, as well as differentiate it from
Hepatitis A, C or D.

None of these stained for copper, such as in Wilson's disease. For that, we
use rhodanine. I believe it did not stain the actual copper, but rather the
protein to which copper was bound.

Let me know what procedures you need.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak MI 48073


Lee & Peggy Wenk
-----Original Message-----
[] On Behalf Of Malam
Sent: Wednesday, August 10, 2005 6:55 AM
To: Histonet submissions (
Subject: [Histonet] Orcein for copper-binding protein/Hep B

Hello Histonetters.
Has anyone got a really good orcein method for copper-binding protein/Hep B,
and is there a recommended brand or vendor of the dye? Would you use
synthetic or natural (if you can still get it)?
Jacqui Malam
Lancaster Infirmary
N England

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