I think the Chemicon autofluorescence removal reagent - whatever they call=20
it, is to get rid of lipofuscins - their website will have the information=20
how it works.

At 11:20 AM 8/18/2005, you wrote:
>Autofluorescence in CNS tissue, as well as some other tissues, is often due
>to the presence of lipofuscin. Staining with Sudan Black B after
>immunolabeling is often effective in suppressing such background
>autofluorescence.
>
>Make up 0.l% solution in 70% ethanol.  Heat to boiling, then cool and
>filter.  Optimum staining time may vary somewhat from one tissue to another
>and for sections of different thickness, but 10 minutes usually provides an
>acceptable level of suppression, and often complete suppression of
>autofluorescence.  Quickly rinse off the stain with water or buffer, several
>rapid changes, and coverslip as usual.
>
>Paul M.
>
> > ----------
> > From:         histonet-bounces@lists.utsouthwestern.edu on behalf of
> > klapka
> > Sent:         Thursday, August 18, 2005 5:33 AM
> > To:   Histonet
> > Subject:      [Histonet] Fluorescence backgound
> >
> > Hello all,
> >
> > does anyone use a method to suppress autofluorescence of tissue due to
> > PFA fixation? I have large background in my PFA fixated nervous tissue...
> > Thank you,
> > Nicole
> >
> > --
> > Nicole Klapka PhD
> > Labor für Molekulare Neurobiologie
> > TVA, Geb. 22.22
> > Heinrich-Heine Universität Düsseldorf
> > Universitätsstr. 1
> > 40225 Düsseldorf
> > Tel: ++49 211 81 14437
> >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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