Patsy, 

The average routine fluorescent system uses "longpass" emission filters that
let almost all the light from blue to red through. Therefore you will see
many colors of  autofluoresce. Since the autofluorescence is usually a
different color than your target fluorchrome, the way you can avoid
autofluoresence is to find filters that block the autofluorescent
wavelength. It takes more effort to coordinate the different wavelengths
associated with the fluorochrome excitation and emission vs the
autofluorescent excitation and emission, but by using narrow-bandwidth
filters (20-40 nm bandwidth) it is usually possible. Both Omega and Chroma
have these filters.

Tim Morken
Lab Vision - Neomarkers
www.labvision.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Friday, August 12, 2005 1:18 PM
To: 'Histonet'
Subject: [Histonet] Flourescent labels


Folks,
I was under the general impression that most flourescent labels were done on
frozen tissue because of problems with auto flourescence associated with
aldehyde fixation and paraffin processing.  Has the technology developed so
that these new flourescent labels such as the Alexa dyes can now be used on
ffpe tissues? Patsy
 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net  
 

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