RE: [Histonet] F4/80 Ab clone CI:A3-1 not working for foam cells - why?

From:"Patsy Ruegg"

F4/80 may not pick up as many of the macrophage like cells as some of the
CD68's.  I really like KPMI CD68.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
wk email
web site

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

-----Original Message-----
[] On Behalf Of GT Hebert
Sent: Friday, August 05, 2005 9:40 AM
Subject: [Histonet] F4/80 Ab clone CI:A3-1 not working for foam cells - why?

Hello again,
I just worked out my F4/80 antibody on mouse tissue.  The mouse tissue,
aortic arch with athrosclerotic lesions came up negative though.  I would
think that the foam cells (macrophages??) would light up as the do when I
stain with CD68 ... but only 1 or 2 cells outside the lesion lit up.  The
Kupffer cells in the liver and the red pulp in the spleen (my + controls)
came out great and the negative isotype controls on all samples are clear.
Any idea why this may be happening?  I have seen this antibody used on a
number of literature papers staining foam cells in AT lesions (same clone -
just different dilutions (1:25, 1:200m etc -- all paraffin) -- and they
showed staining of foam cells.  
My tissues are all treated the same: 24 hour fixation in 4%PF, routine
processed and embedded, 4 micron sections, deparaffinized ... and then IHC
(optimal conditions).
I'm sort of lost with this one ... I know the antibody works.  Just the
results are not what I expected.  Any information would be greatly
Gustave Hebert
Wyeth Cambridge

 Start your day with Yahoo! - make it your home page
Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>