I often combine the Verhoeff Elastin Stain with the Gomori Trichrome.  I do
the Bouin's treatment required for the trichrome procedure first.  Then I do
the complete Verhoeff stain, eliminatiing the final Van Gieson counterstain.
Then I apply the one-step Gomori Trichrome. It is quite simple and gives
excellent results.

Paul M.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of GT
> Hebert
> Sent: 	Tuesday, August 2, 2005 6:05 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] Elastic Trichrome - any good protocol out there
> not involving microwave???
> 
> Hello all,
>  
> I am in need of a good protocol for a combination elastic and trichrome
> stain that doesn't involve microwave.  Thanks.
>  
> Gustave Hebert
> Scientist II
> Wyeth Research
> Cambridge MA
> 
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