Question about Re: [Histonet] EDTA recipe with PVP

From:Gayle Callis


What is the advantage of adding PVP to the EDTA decalcifying 
solution?  Just curious.

At 05:38 AM 8/26/2005, you wrote:
>Here is our recipe for EDTA containing PVP:  Dissolve 41.3 grams EDTA 
>(disodium salt), 4.4 grams Sodium Hydroxide and 50 g PVP-40 (Sigma) in 
>1000 ml distilled water over very low heat.  Cool to room temperature, 
>adjust pH to 7.4 once cooled.  We use this to gently decalcify our mouse 
>long bones, it will take about 3 weeks to decalcify an adult mouse femur.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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