[Histonet] rat heart cryos' for immunos
Does anyone know why heart cryos tend to separate? We've tried fresh frozen, NBF perfused, sucrose perfused and FBS perfused and the heart tissue has 'holes' in it.
Laurie L. Pereira, RVT
SDCADDL (County Veterinarian)
5555 Overland Avenue Suite 4103
San Diego, CA. 92123
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