[Histonet] (no subject)
I have used a setup similar to the one described in the previous
reply by Dr. Chiovetti, using a coolpix 4500 camera.
You can set a zoom of the coolpix objective to a precise numerical
value using the "photopc" freely downloadable software and a serial
cable (not the USB cable supplied with the camera; Coolpix serial
cable can be purchased for ` $25).
There are DOS, windows and Linux versions of the software.
I set up an old computer as a linux system and wrote a simple shell
script that would on startup set the zoom of the camera to a certain
default value (say 12.7 mm), as well as set some other parameters.
The computer also serves as a remote release, so that you do not have
to spend another $80 for the remote release gadget and just press the
"Enter " key to take a picture.
If you are interested, I will be happy to send you the shell script
and more info on 'photopc" software directly as an attachment.
>Date: Mon, 15 Aug 2005 11:38:55 EDT
>Subject: Re: [Histonet] Nikon relay lens
>To: email@example.com, firstname.lastname@example.org
>Content-Type: text/plain; charset="US-ASCII"
>In a message dated 8/14/2005 11:35:35 PM US Mountain Standard Time,
> > We're using an Olympus BX 50 light microscope attached with Nikon Coolpix
> > 5000 digital camera. I'm wondering what the mangnification value
> of MDC relay
> > lens between the camera and the microscope is.
>4. Since the zoom on the camera is infinitely adjustable, we had to decide
>on a reproducible zoom setting that always gave the same magnification. For
>the Coolpix 8800 and our adapter, we opted to zoom to the maximum
>10x on the 8800; I'm not sure what it is on the 5000.
>5. To make measurements we set the camera to this maximum (10x) zoom, and
>took pictures of the stage micrometer with each objective. From there, it's
>relatively easy to measure the spacing of the 10 micrometer lines and to
>calculate a total magnification.
>This was the only way we could come up with to make measurements, since the
>camera's zoom is so variable. It's a small inconvenience, but the
>great with this set-up.
>You could probably use a similar procedure, provided you are using the
>camera's optical zoom to fill the field of view with the MDC relay
>lens. The trick
>is to use a zoom setting that is reproducible.
>Hope this is of some help.
>Robert (Bob) Chiovetti, Ph.D.
>The Microscope Works
>Arizona's Microscopy Resource
>132 North Elster Drive
>Tucson, AZ 85710-3212 USA
>Member, Arizona Small Business Association - ASBA
Dr. Stanislav Vitha email@example.com
Microscopy and Imaging Center
Texas A&M University
College Station, TX 77843-2257
tel: 979-845-1129 (main desk)
tel: 979-845-1607 (direct link)
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