Hello all,

I have been struggling with high background in frozen mouse sections
processed for immunofluorescence.  A colleague told me that switching from
PBS to a Tris buffer reduced their non-specific background.  Does anyone
know why this would make a difference?  Also, I have tried using 1% sodium
borohydride for my paraformaldehyde fixed sections, but the slide mounted
tissue trapped gas bubbles and made it wrinkly.  Is there a way to avoid the
bubbles under the tissue?

Thanks!

 

Carolyn Johnson

Research Assistant

The Rockefeller University

1230 York Ave, Box 275

New York, NY 10021

Phone:(212) 327-8672

Fax:(212) 327-8664

  cjohnson@rockefeller.edu

 

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