[Histonet] can PBS cause background?
I have been struggling with high background in frozen mouse sections
processed for immunofluorescence. A colleague told me that switching from
PBS to a Tris buffer reduced their non-specific background. Does anyone
know why this would make a difference? Also, I have tried using 1% sodium
borohydride for my paraformaldehyde fixed sections, but the slide mounted
tissue trapped gas bubbles and made it wrinkly. Is there a way to avoid the
bubbles under the tissue?
The Rockefeller University
1230 York Ave, Box 275
New York, NY 10021
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