[Histonet] brain sections lifting


Hi I have a student who is doing fluorescent immunochemistry and has
problems with her sections lifting off her slides. We are using aptes 
coated slides but they are still lifting. I have told her to make sure
the sections are as flat as possible when she picks them up from the
waterbath but we still have a problem. She is also leaving them
overnight at 60 degrees. Does anybody have any suggestions. I thought of
using chrome gelatin but I am not sure if this is going to help.
Sharon Marshall
Dept. Human Biology
University of Cape Town
South Africa
e-mail: marshall@cormack.uct.ac.za

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