[Histonet] Sihler's stain

From:"Dianne Holmes"

I have been asked to use the Sihler technique to stain nerves in the trachea.  I have only 'heard' of this method and would like some input from someone who has actually used  it.  The reprint I was given to refer to is a 2001 experiment on rabbit eyes and it took up to 3 months to process the tissue !!  Any help would be appreciated.

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