[Histonet] Re: rat on rat

From:Gayle Callis

No problems - but you would have rat on rat problems if you were using a 
rat host primary i.e. Rat antiRat primary.

You can use a donkey or goat antiMouse secondary adsorbed to rat tissues, 
preferably an F(ab')2 frag of IgG - Jackson has excellent secondaries for 
this.  We used a goat antiMouse isotype i.e IgG1, 2, etc -biotin conjugate 
from Southern Biotechnology.  The latter was very clean and I strongly 
recommend these.

Just do normal serum blocking carefully - you could even add 1% rat serum 
to the normal serum block with either goat or donkey serum but I don't 
think that will be needed.

At 12:45 PM 8/2/2005, you wrote:
>Any suggestions? The antibody is a mouse anti-rat and it is to be used
>on rat tissue. Does that present the same problems like mouse on mouse?
>If so, what do you can you do about it?
>Renee' Till, HT
>Arkansas Childrens Nutrition Center
>1120 Marshall
>Little Rock, AR
>(501) 364-2774
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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