[Histonet] Re: fixation of fresh frozen section

From:Gayle Callis


What are you staining for, i.e. what antigens?  That determines what 
fixative we choose to use.

At 07:39 AM 8/3/2005, you wrote:
>Hi all
>When I do immunocytochemistry on fresh frozen section I fix most, if not
>all slides with acetone. Sometimes I use cold methanol because the
>datasheet tells me to do so. Is anyone of you who prefer other
>Joost Bruijntjes
>TNO Quality of Life

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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