You can use either air-dried or alcohol fixed smears. Both work fine. If you are looking for cryptococcus and using a mucicarmine stain, then the alcohol looks better. GMS and PAS, I also prefer alcohol fixed, but the air-dried also works if that is all you have.

Elizabeth Martin, M.D.
Northern Virginia Community Hospital
Arlington, VA


Today's Topics:

   1. Re: Stain for fungi in cytological smear (Matt Prideaux)


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Message: 1
Date: Wed, 24 Aug 2005 17:34:44 +0100
From: Matt Prideaux 
Subject: Re: [Histonet] Stain for fungi in cytological smear
To: RStaskiewicz@agr.state.il.us, Histonet
	
Message-ID: <1124901283.430ca1a401829@webmail.shef.ac.uk>
Content-Type: text/plain; charset=ISO-8859-1

The lab I used to work at did Grocott's staining for fungi on cytology smears
regularly, without the cells washing off. I think the slides were APES
treated.

Hope that helps.

-- 
Matt Prideaux
Academic Unit of Bone Biology
Division of Clinical Sciences (South)
D Floor (DU20) Medical School
Henry Wellcome Laboratories for Medical Research
University of Sheffield
Beech Hill Road
Sheffield 
S10 2RX


Quoting RStaskiewicz@agr.state.il.us:

> Good morning all! I have a request to stain a cytological smear of a nasal 
> wash for cryptococcus. I have never stained smears for fungi, just tissue. 
> Does anyone have any experience or suggestions?
> 
> 
> Rae Ann Staskiewicz HT(ASCP)
> Section Head-Histology
> Galesburg Animal Disease Lab
> 2100 S. Lake Storey Rd
> Galesburg, IL 61401
> (309) 344-2451
> rstaskiewicz@agr.state.il.us
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



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