Jim,

...perhaps it is "sodium azide" rather than sodium nitride????  

Hydrogen peroxide and sodium azide together in solution is very
effective in eliminating "endogenous peroxidase"

 

Robert

 

Robert L. Lott, HTL(ASCP)

Baptist Health System

800 Montclair Road

Birmingham, AL   35213

205-592-5388  phone

205-592-5646  fax

robert.lott@bhsala.com

 

 

From:       histonet-bounces@lists.utsouthwestern.edu on behalf of Mr

James Hugh Reilly

 Sent:      Tuesday, August 16, 2005 12:28 AM

 To: histonet@lists.utsouthwestern.edu

 Subject:   [Histonet] Endogenous Peroxidase

 

 I have been asked to try a new Immumohistochemistry (IHC) technique
which

 uses 1% hydrogen peroxide and 2% sodium nitride in phosphate buffer

 solution.  Does anyone know what sodium nitride Is? and where can it be

 purchased?

 

 Jim Reilly

 

 University Dept. Medicine

 Level 3 QEB

 Royal Infirmary

 10 Alexandra Parade

 Glasgow

 G31 2ER

 

 

 

Message: 10

Date: Tue, 16 Aug 2005 09:41:57 -0400

From: "Monfils, Paul" 

Subject: RE: [Histonet] Endogenous Peroxidase

To: "'histonet@lists.utsouthwestern.edu'"

      

Message-ID:

 
<09C945920A6B654199F7A58A1D7D1FDE017175AE@lsexch.lsmaster.lifespan.org>

      

Content-Type: text/plain;     charset="iso-8859-1"

 

Sodium nitride is Na3N, but I don't think you will be able to purchase
it

anywhere.  It is a compound that was long believed to be chemically

impossible, but which only recently was experimentally synthesized for
the

first time by deposition of atomic sodium and nitrogen beams onto a

supercooled sapphire in a vacuum.  I doubt that the compound is
commercially

available.  Perhaps it is sodium nitrite that is called for in your

protocol?  

 

Paul M.

 

 

 

> ----------

> From:     histonet-bounces@lists.utsouthwestern.edu on behalf of Mr

> James Hugh Reilly

> Sent:     Tuesday, August 16, 2005 12:28 AM

> To:       histonet@lists.utsouthwestern.edu

> Subject: [Histonet] Endogenous Peroxidase

> 

> I have been asked to try a new Immumohistochemistry (IHC) technique
which

> uses 1% hydrogen peroxide and 2% sodium nitride in phosphate buffer

> solution.  Does anyone know what sodium nitride Is? and where can it
be

> purchased?

> 

> Jim Reilly

> 

> University Dept. Medicine

> Level 3 QEB

> Royal Infirmary

> 10 Alexandra Parade

> Glasgow

> G31 2ER

> 

> 

> _______________________________________________

> Histonet mailing list

> Histonet@lists.utsouthwestern.edu

> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

> 

 

 

 

------------------------------

 

Message: 11

Date: Tue, 16 Aug 2005 09:51:48 -0400

From: "John A. Kiernan" 

Subject: Re: [Histonet] Endogenous Peroxidase

To: Mr James Hugh Reilly 

Cc: histonet@lists.utsouthwestern.edu

Message-ID: <4301EF74.DCDF1755@uwo.ca>

Content-Type: text/plain; charset=us-ascii

 

I think there must be a mistake in the name.

Sodium nitride (Na3N) is a grey solid formed by

heating metallic sodium in nitrogen. It is

instantly decomposed by water, so a solution in

phosphate buffer could not be made. 

-- 

-------------------------------

John A. Kiernan

Department of Anatomy and Cell Biology

The University of Western Ontario

London,   Canada   N6A 5C1

   kiernan[AT]uwo.ca

   http://publish.uwo.ca/~jkiernan/

   http://instruct.uwo.ca/anatomy/530/index.htm

_______________________________

Mr James Hugh Reilly wrote:

> 

> I have been asked to try a new Immumohistochemistry (IHC) technique
which uses 1% hydrogen peroxide and 2% sodium nitride in phosphate
buffer solution.  Does anyone know what sodium nitride Is? and where can
it be purchased?

> 

> Jim Reilly

> 

> University Dept. Medicine

> Level 3 QEB

> Royal Infirmary

> 10 Alexandra Parade

> Glasgow

> G31 2ER

> 

> _______________________________________________

> Histonet mailing list

> Histonet@lists.utsouthwestern.edu

> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 

 

 

------------------------------

 

Message: 12

Date: Tue, 16 Aug 2005 09:52:14 -0400

From: "Smith, Allen" 

Subject: RE: [Histonet] Endogenous Peroxidase

To: "Mr James Hugh Reilly" 

Cc: histonet@lists.utsouthwestern.edu

Message-ID:

 
<5D2189E74151CC42BEC02906BA8996322B90DD@exchsrv01.barrynet.barry.edu>

Content-Type: text/plain;     charset="us-ascii"

 

Sodium nitride, Na3N, is a rather unstable compound first prepared in
2002.

The synthesis is notoriously difficult.  I do not think it is
commercially

available.  I think that the "nitride" in your recipe is a typographical

error in "nitrite".  Sodium nitrite is available from both Aldrich and

Fisher.

 

Allen A. Smith, Ph.D.

Professor of Anatomy

Barry University School of Graduate Medical Sciences

    Podiatric Medicine and Surgery

Miami Shores, Florida  33161

 

 

-----Original Message-----

From: histonet-bounces@lists.utsouthwestern.edu

[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mr James

Hugh Reilly

Sent: Tuesday, August 16, 2005 3:29 AM

To: histonet@lists.utsouthwestern.edu

Subject: [Histonet] Endogenous Peroxidase

 

I have been asked to try a new Immumohistochemistry (IHC) technique
which

uses 1% hydrogen peroxide and 2% sodium nitride in phosphate buffer

solution.  Does anyone know what sodium nitride Is? and where can it be

purchased?

 

Jim Reilly

 

University Dept. Medicine

Level 3 QEB

Royal Infirmary

10 Alexandra Parade

Glasgow

G31 2ER

 

 

 

 



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