[Histonet] Dried-out muscle biopsy
I hoping someone can help me troubleshoot frozen muscle biopsies that are
sometimes extremely difficult to cut because it's so dried out. The muscle
looks like it would be easy to cut, but ends up being difficult and sections
hard to get. What would "dry-out" muscle tissue? Every now and then, some
muscle biopsies are so dried out that they seem like powder coming off the
cutting blade. Three things come to mind as possible reasons: Specimen dried out
shortly after procedure and transport, during freezing the muscle, or the
cryostat is too cold.
Thanks for thoughts and responses!
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