[Histonet] Cryostat sectioning of mammary gland

From:"Tessa Murray"


   I'm looking for some advice on cryosections.  We are trying to collect
   frozen  sections of mammary gland tissue and are experiencing problems
   - we do a lot of paraffin work here so the cryostat technique is quite
   new to us.  We find that the knife cuts the OCT fine, but when it hits
   tissue  it does not cut so we get sections with tissue shaped holes in
   them.   We've  tried  changing the block and knife temp (we have tried
   altering  them  between  -30  to -35C) but nothing seems to help.  Any
   advice appreciated...

   tess
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