[Histonet] Concentration of detergents used with Ventana Fish
Gudrun,
It seems to me that Ventana technical services can and should supply you
with this information i.e. percentage of Tween 20 or Triton X 100 or is it
on the MSDS (Material Safety Data Sheets) for the Reaction buffer?
At 07:06 AM 8/8/2005, you wrote:
>Hi Gudrun,
>We are currently running Her2 Fish on our Ventana with 100% correlation
>with other vendor's chemistries.
>This is what works very well in my lab:
>1)Under subdued lighting, I remove the slides and place them in 2
>changes Reaction buffer.
>2)Soak in the second change of reaction buffer for 5 minutes. I believe
>there is some kind of surfactant in the Reaction buffer that does the
>trick.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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