[Histonet] Concentration of detergents used with Ventana Fish

From:Gayle Callis


It seems to me that Ventana technical services can and should supply you 
with this information i.e. percentage of Tween 20 or Triton X 100 or is it 
on the MSDS (Material Safety Data Sheets) for the Reaction buffer?

   At 07:06 AM 8/8/2005, you wrote:
>Hi Gudrun,
>We are  currently running Her2 Fish on our Ventana with 100% correlation
>with other  vendor's chemistries.
>This is what works very well in my lab:
>1)Under subdued lighting, I remove the slides and place them in 2
>changes Reaction buffer.
>2)Soak in the second change of reaction buffer for 5 minutes. I believe
>there is some kind of surfactant in the Reaction buffer that does the

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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