[Histonet] Apoptosis detection in plastic embedded tissue
|From:||"Eijl, drs. T.J.A. van" |
We have been trying to stain apoptotic cells in Carnoy fixed, BMA/MMA embedded mouse lungs using the chromogenic DEAD-END Kit by Promega. However, this kit has been designed for formalin fixed, parafin embedded tissue. To cut a long story short, we did not get it to work properly.
Now I am enquiring if anybody has knowledge of an apoptosis detection method that can be used on plastic embedded tissues as described above.
Thanks in advance,
Sven van Eijl
Dept. of Pharmacology & Pathophysiology
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