Re: [Histonet] special stains on slides that have been to waterovernight

From:"Bryan Hewlett"

Hi Diana,

Provided that the tissues were NBF fixed for a minimum of 24 hours, you
should have NO problem with the common special stains and with the immuno
stains.
In fact, back at the beginning of IHC techniques this was a fairly standard
procedure with us and provided a measure of antigen retrieval for many
difficult antigens( basically it's AR at RT!).

Been there, done that!

Regards,

Bryan

----- Original Message -----
From: "Diana McCaig" 
To: 
Sent: Wednesday, August 18, 2004 5:56 PM
Subject: [Histonet] special stains on slides that have been to
waterovernight


> I was hoping I could get some advice regarding an issue.  Would a special
> stain or immuno stain on a section that has been brought to water and left
> to sit overnight in water yield reliable results.  It gets cut, dried and
> taken to water the previous day and the staining procedure is not carried
> out until the next day.  Your opinions would be appreciated.
> Diana McCaig,
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>