It is my experience that initial fixation in alcohol can affect
immunoreactivity for those proteins that are highly soluble (e.g. S-100
and calretinin).  We also have a difficult time identifying
synaptophysin and chromogranin in alcohol-fixed cytology specimens.  We
have seen several specimens where the cytology preparations have been
negative, but tissue submitted to histology has been positive.

Richard



Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Browning Deb  08/26/04 09:59AM >>>
I would like to know how many institutions make an effort to produce
cell
blocks from FNA specimens, and whether the 50% alcohol that is used to
collect the FNA affects any IHC results.  Thanx

Debra Browning, ART
Technical Specialist, Immunohistochemistry
Anatomic Pathology
Hamilton Health Sciences
phone: (905) 527-4322  ext 46131
e-mail:  browning@hhsc.ca 
fax: (905) 524-2681


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