Re: [Histonet] Using Cy2 immunofluorescence?
For a biotinylated primary, you should come back with Strepavidin
conjugated to the CY2 fluorophore. I don't think the secondary conjugated
to Cy2 will like all that biotin in the way when you try to connect primary
to secondary. Jackson Immunoresearch sells SA-Cy2, not terribly expensive
and they have excellent products.
If you use a biotinylated primary, be sure to use an avidin/biotin block or
better yet, Strepavidin/biotin blocking kit (both available from Vector).
At 09:02 AM 8/26/2004, you wrote:
>We are using a cy2 immunofluorescent secondary antibody for IHC, and
>we would like to know if there are any complications using it on a
>biotin conjugated primary. We are not sure if there will be
>interferance with the signal or if there will be no sites on the
>primary ab for the secondary to bind. I appreciate your help.
>University at Buffalo
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
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