Re: [Histonet] T blue for mucin veronal acetate buffer

From:Geoff McAuliffe

Hi Kathy:

    You don't need veronal acetate buffer to get good tol. blue staining 
of GAGs, other acetate buffers (check the buffer tables at the back of 
most histotechnique books) will work just as well. Pick the pH you want 
and go for it.
    Metachromasia can sometimes be retained if you dehydrate in acetone 
rather than alcohol, then clear in fresh xylenes (w/o previous 
contamination w/ alcohols).


Kathleen Cormier wrote:

> Hello All!
> I am looking for a supplier for the veronal acetate buffer needed in 
> the T blue for mucin (Theory and Practice ver 1980 pg 169-70) Since 
> the buffer uses sodium barbiturate (alas! a controlled substance!) it 
> would be much easier to get this buffer pre made. Any one know of 
> anyone who sells this buffer or kit? Is there a similar stain that 
> does not have sodium barbiturate? Is this a lost cause? This 
> researcher is looking at gel membranes with glycosaminoglycans chains, 
> and would like the T blue to stain the sulfated  and  nonsulfated 
> mucopolysaccharides...
> Thanks!
> Kathy
> _______________________________________________
> Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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