Re: [Histonet] Substitute fixative for B5

From:"Richard Cartun"

After eliminating B5 several years ago we experimented with several
different fixatives.  We ended up going back to formalin and, as long as
the specimen is adequately fixed, the morphology has been excellent.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Prestridge, Linda"  08/16/04 12:00PM >>>
We are trying to quit using B5.
We are did study with B-Plus from BBc Chemical, IBF from Surgipath,
Alcoholic Zinc from ANatech and AZF from Newcomers.
Well the pathologists ruled it down to B-Plus and AZF from Newcomers.
We then did battery of immuno's - Lymphoma panel. Most stained fair,
they narrowed it down to B-Plus.
But...the problem is that I can't get the kappa and lambda (poly's) to
stain. I tried AR and got some staining but very dirty.
What are any of you using and have you had the same problem getting
kappa and lambda to stain???
Thanks, Linda
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