Re: [Histonet] MMP-1 immuno labelling of placenta
|From:||"SMITH,REBEKAH FELICIA" |
I've been doing eNOS staining on placenta and I found it helpful
to block in 1:10 30%peroxide to methanol for 1hr (to get rid of
endogenous peroxidase-I know most people probably don't do it for
that long though, but shorter times didn't help) and NaOH for 15
min to deal with the blood cells staining.Good luck! I feel your
On Mon Aug 16 11:30:51 EDT 2004, "Y. Wang"
> Hello Louise,
> Thank you for your suggestions.
> 1) Seeing as we could not go to the farm to collect the tissue
> the tissue
> was kept on ice (~5 hours) while it was shipped to us and
> embedded. Do you think this is a problem?
> 2)We have not done any biotin blocking for these samples. Thank
> you for
> suggesting that. We will try this next.
> Thanks again
> Yak-Nam Wang
> On Mon, 16 Aug 2004, renton louise mrs wrote:
>> 1. Was the tissue fresh when sampled? autolysis can alter IHC
>> 2.Placenta contains a lot of biotin...are you blocking
>> -----Original Message-----
>> From: "Y. Wang"
>> To: Histonet@lists.utsouthwestern.edu
>> Date: Fri, 13 Aug 2004 14:35:07 -0700 (PDT)
>> Subject: [Histonet] MMP-1 immuno labelling of placenta
>> Has anyone had any experience immuno labelling placenta tissue
>> anti-MMP1? We have tried this (cryosections with ABC kit with
>> DAB) but are
>> getting alot of (what we assume to be) background and nothing
>> that looks
>> like images we have seen on-line. Seeing as we have not had any
>> with placenta tissue we are questioning if we are took the
>> tissue from the
>> correct place from the placenta (we were given a whole one which
>> we took
>> random samples from).
>> Thank you for your help
>> Yak-Nam Wang
>> Senior Fellow
>> Department of Bioengineering
>> University of Washington
>> Box 357962
>> Seattle, WA 98195
>> Tel.: (206)-221-5873
>> Fax.: (206)-221-5874
>> Histonet mailing list
>> Louise Renton
>> Bone Research Unit
>> University of the Witwatersrand
>> South Africa
>> .......so what IS the speed of dark?
>> Histonet mailing list
> Histonet mailing list
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