Hello Louise,

Thank you for your suggestions.
1) Seeing as we could not go to the farm to collect the tissue the tissue
was kept on ice (~5 hours) while it was shipped to us and immediately
embedded. Do you think this is a problem?
2)We have not done any biotin blocking for these samples. Thank you for
suggesting that. We will try this next.

Thanks again
Yak-Nam Wang


On Mon, 16 Aug 2004, renton louise mrs wrote:

> 1. Was the tissue fresh when sampled? autolysis can alter IHC dramatically
> 2.Placenta contains a lot of biotin...are you blocking adequately?
>
> Regards
>
>
>
> -----Original Message-----
> From: "Y. Wang" 
> To: Histonet@lists.utsouthwestern.edu
> Date: Fri, 13 Aug 2004 14:35:07 -0700 (PDT)
> Subject: [Histonet] MMP-1 immuno labelling of placenta
>
> Hello,
>
> Has anyone had any experience immuno labelling placenta tissue with
> anti-MMP1? We have tried this (cryosections with ABC kit with DAB) but are
> getting alot of (what we assume to be) background and nothing that looks
> like images we have seen on-line. Seeing as we have not had any experience
> with placenta tissue we are questioning if we are took the tissue from the
> correct place from the placenta (we were given a whole one which we took
> random samples from).
>
> Thank you for your help
> Yak-Nam Wang
>
> Senior Fellow
> Department of Bioengineering
> University of Washington
> Box 357962
> Seattle, WA 98195
>
> Tel.: (206)-221-5873
> Fax.: (206)-221-5874
>
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>
>
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> .......so what IS the speed of dark?
>
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