Re: [Histonet] IHC polymer detection system for rat tissues

From:Patti Loykasek

I had an email today from my Signet rep that they have a new mouse on mouse
polymer kit. I have not tried it, but you may want to contact them.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA>

 Has anyone found a successful polymer detection system (i.e., Envision or
> others) directed against a mouse monoclonal on rat tissue. We tried one but
> got a lot of background staining, I suspect from cross reactivity with
> closely related antigens. Our standard IHC approach uses an anti-mouse,
> rat-absorbed secondary antibody, but I would like to get the added
> sensitivity of a polymer-based system.
> Ronald P. Wilson, V.M.D., M.S.
> Associate Professor
> Department of Comparative Medicine, HO54
> Penn State University College of Medicine
> M. S. Hershey Medical Center
> 500 University Drive
> Hershey, PA 17033
> phone:  717-531-8460
> fax:       717-531-5001
> e-mail:
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