RE: [Histonet] tris-HCl antigen retrieval

From:"IHC World"

IHC World website has many different antigen retrieval protocols including
Tris-HCl pH10. Here is the link to the protocols: Hope this helps.

IHC World
Online Information Center for Immunohistochemistry

-----Original Message-----
From: []
Sent: Tuesday, August 17, 2004 2:55 PM
Subject: [Histonet] tris-HCl antigen retrieval

Would any of you be so kind as to share any and all formulae for buffers
utilizing Tris base and HCl for antigen retrieval, with a pH of 10.0?  I
have one formula but it is giving me uneven results.
Thanks in advance,
Nancy Lemke
Hermelin Brain Tumor Center
Henry Ford Hospital
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