Jim,
That slide will work fine for special stains.
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Crainiofacial Research
Neuronal Gene Expression Section

> ----------
> From: 	Jim Staruk
> Sent: 	Wednesday, August 18, 2004 7:05 PM
> To: 	'Diana McCaig'; histonet@lists.utsouthwestern.edu
> Subject: 	RE: [Histonet] special stains on slides that have been to
> waterovernight
> 
> I tried that once and the immuno was a total failure the next day.  I
> never investigated it as to why, assuming everything washed out
> overnight.
> 
> Jim
> 
> ________________________
> James E. Staruk, HT(ASCP)
>   www.masshistology.com
>  
>  
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana
> McCaig
> Sent: Wednesday, August 18, 2004 4:56 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] special stains on slides that have been to
> waterovernight
> 
> I was hoping I could get some advice regarding an issue.  Would a
> special
> stain or immuno stain on a section that has been brought to water and
> left
> to sit overnight in water yield reliable results.  It gets cut, dried
> and
> taken to water the previous day and the staining procedure is not
> carried
> out until the next day.  Your opinions would be appreciated. 
> Diana McCaig,
> 
> 
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