Check the mountant you are using. Some will fade Spirochete silver stains
very quickly

Regards,

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] 
Sent: Wednesday, 18 August 2004 2:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] silver question


All:
 
Has anyone experienced a problem with their Steiner's "fading" after a
period of time?  We had a slide that had been stained with the Steiner
procedure and it was very positive, however when it was reviewed at a
much later date it appeared negative.  Another section was stained and
it was positive.  I know that leaving the sections for a long time in
xylene before they are coverslipped can lead to fading but wouldn't this
be apparent when the slides were originally viewed?  
 
Any suggestions?
 
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet