RE: [Histonet] fixatives
If you mean a fixative that is also an electron stain, potassium
permanganate (Luft,1956, J. Biophys. Biochem. Cytol. 2:799) and sodium
permanganate (Wetzel, 1961, J. Biophys. Biochem. Cytol. 9:711) have been
uses with some success. While both are safer than osmium tetroxide, neither
gives good results as consistently.
If you just want a fixative for electron microscopy, Karnovsky's (1965)
paraformaldehyde-glutaraldehyde mixture is one of the best (J. Cell Biol.
27:137A or any EM handbook). Plain glutaraldehyde also works: any EM
handbook will give a formula for 2.5% glutaraldehyde in pH 7.2-7.4 phosphate
or cacodylate buffer. (Note that cacodylate is very poisonous.)
Allen A. Smith, Ph.D.
Professor of Anatomy
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida
[mailto:firstname.lastname@example.org] On Behalf Of pam plumlee
Sent: Friday, August 27, 2004 12:05 PM
To: HistoNet Server
Subject: [Histonet] Coverslipping tape
Hi all: I know this has been asked before, is there a
quick fix for removing tissue from coverslipping tape,
reattaching to the slide, covering with a new glass
coverslip so the tissue can be photographed? Also,
does anyone know a good alertnate fixative for osmium
tetroxide? We are trying to keep it out of the lab.
We have been asked to use it for a post-fix for fatty
tissue and on an EM processor. Thanks, Pam
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