Linda,

We have also used a 2% agarose solution and have been sucessful embedding
the agarose-cell mixture in OCT for frozen sections.

Yak-Nam

Senior Fellow
Department of Bioengineering
University of Washington
Box 357962
Seattle, WA 98195

Tel.: (206)-221-5873
Fax.: (206)-221-5874

On Wed, 18 Aug 2004, Elizabeth Chlipala wrote:

> Linda
>
> I use a 2% agarose solution.  Basically, you spin the cells down, remove
> the supernatant and then re-suspend in the agarose and spin again.  You
> can remove the agarose pellet, process and embed in paraffin.  I'll send
> you the pdf of the reference I have used.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Histology Laboratory, LLC
> P.O. Box 18592
> Boulder, Colorado 80308
> Office: (303) 735-5001
> Fax: (303) 735-3540
> lizchlipala@premierhistology.com
> www.premierhistology.com
>
> Ship to Address:
> Premier Histology Laboratory
> University of Colorado
> MCBD, Room A3B40
> Boulder, Colorado 80309
>
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> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
> MARGRAF
> Sent: Wednesday, August 18, 2004 1:29 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] fibrin for cell blocks
>
> Dear Histonetters:
> Does anyone have experience using fibrin or another substance as a
> matrix to add a cell suspension to when  making cell blocks.  My
> colleague wants to make cell blocks to section and stain for a research
> study but there will only be a small amount of cellular material.  She
> remembers seeing this done in the past but needs to know where to obtain
> the reagnets and what procedures to follow. Thanks so much
> Linda M
> Histonet administrator
>
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