Linda

I use a 2% agarose solution.  Basically, you spin the cells down, remove
the supernatant and then re-suspend in the agarose and spin again.  You
can remove the agarose pellet, process and embed in paraffin.  I'll send
you the pdf of the reference I have used.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Wednesday, August 18, 2004 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fibrin for cell blocks

Dear Histonetters: 
Does anyone have experience using fibrin or another substance as a
matrix to add a cell suspension to when  making cell blocks.  My
colleague wants to make cell blocks to section and stain for a research
study but there will only be a small amount of cellular material.  She
remembers seeing this done in the past but needs to know where to obtain
the reagnets and what procedures to follow. Thanks so much
Linda M
Histonet administrator

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