RE: [Histonet] IHC polymer detection system for rat tissues

From:"Elizabeth Chlipala"


We have been able to amplify rat anti mouse antibodies with a polymer
based system such as Envision.  In this case we use a rabbit anti-rat
(mouse adsorbed) secondary and then we amplify by using the rabbit
envision instead of strep ABC or ABC.  I'm assuming that this can be
done with mouse antibodies if there is a secondary antibody of rabbit
anti-mouse that can be used to link the rabbit envision to your primary.
I hope this helps


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
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Premier Histology Laboratory
University of Colorado
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Boulder, Colorado 80309
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-----Original Message-----
[] On Behalf Of Ronald
P. Wilson
Sent: Thursday, August 19, 2004 12:16 PM
To: Histonet
Subject: [Histonet] IHC polymer detection system for rat tissues

Has anyone found a successful polymer detection system (i.e., Envision
others) directed against a mouse monoclonal on rat tissue. We tried one
got a lot of background staining, I suspect from cross reactivity with
closely related antigens. Our standard IHC approach uses an anti-mouse,
rat-absorbed secondary antibody, but I would like to get the added
sensitivity of a polymer-based system.

Ronald P. Wilson, V.M.D., M.S.
Associate Professor
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001

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