Generally speaking, RPMI is highly physiologic, so skin punch bx may be fine
-- especially if refrigerated in the interim.  However, RPMI also readily
supports microbial growth.  If that latter occurred in this instance, bx may
be useless.

Gary Gill

-----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com] 
Sent: Monday, August 30, 2004 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IF on RPMI tissue



All,

I have a client that wants to do an Immunofluorescent Study on a skin punch
bx. that's been in RPMI medium for 5 days.  

Does anyone have any thoughts as to whether or not this will work?  Can
anyone give me an idea on what effect the RPMI may have on the results?

Thanx In Advance,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Coordinator
Milwaukee, WI

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