Do the alcian blue first.
The acid in the alcian blue solution and the rinse will extract hematoxylin.
Nothing other than trifluoroacetic acid (in methylene chloride) can extract
alcian blue.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jose Luis
Palazon Fernandez
Sent: Monday, August 30, 2004 5:02 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] H&E/Alcian Blue



Dear all



I would like to perform an H&E/Alcian blue staining to differentiate bone
and cartilage and I was wondering if I have to do the H&E first and then AB
or the contrary or maybe there is a protocole to this staining technique.
The acid AB solution will bleach the Hematoxylin?. If the AB is used first
why not call the technique AB/H&E instead of H&E/AB?



thanks in advance



jose luis





Universidad de Oriente-Isla Margarita-Venezuela

actualmente en: Instituto de Ciencias Marinas de Andalucia

Puerto Real, Cádiz, España.

email: jluis.palazon@icman.csic.es

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