RE: [Histonet] H&E/Alcian Blue

From:"Joe Nocito"

we perform the Alcian Blue first, then use the H & E as a counterstain. We
haven't had any problems with the staining.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
[]On Behalf Of Jose Luis
Palazon Fernandez
Sent: Monday, August 30, 2004 4:02 AM
Subject: [Histonet] H&E/Alcian Blue

Dear all  I would like to perform an H&E/Alcian blue staining to
differentiate bone and cartilage and I was wondering if I have to do the H&E
first and then AB or the contrary or maybe there is a protocole to this
staining technique. The acid AB solution will bleach the Hematoxylin?. If
the AB is used first why not call the technique AB/H&E instead of H&E/AB?
thanks in advance  jose luis   Universidad de Oriente-Isla
Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de
Andalucia Puerto Real, Cádiz, España. email:

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