Jose,
we perform the Alcian Blue first, then use the H & E as a counterstain. We
haven't had any problems with the staining.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jose Luis
Palazon Fernandez
Sent: Monday, August 30, 2004 4:02 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] H&E/Alcian Blue


Dear all  I would like to perform an H&E/Alcian blue staining to
differentiate bone and cartilage and I was wondering if I have to do the H&E
first and then AB or the contrary or maybe there is a protocole to this
staining technique. The acid AB solution will bleach the Hematoxylin?. If
the AB is used first why not call the technique AB/H&E instead of H&E/AB?
thanks in advance  jose luis   Universidad de Oriente-Isla
Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de
Andalucia Puerto Real, Cádiz, España. email: jluis.palazon@icman.csic.es

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