We have the same problem- and we just recut the slides as they are needed
for send out or review boards.  The important thing is to go back to glass
ASAP.  We use the automatic glass coverslippers now.
Sorry to hear about your predicament, I know it's  a rude situation to
confront.   -Janet
 
-----Original Message-----
From: Coulter, Diane
To: 'histonet@lists.utsouthwestern.edu'
Sent: 8/31/2004 10:29 AM
Subject: [Histonet] Coverslipping tape problems

We're having serious problems with older (7-8 yrs) slides coverslipped
with
tape.  When these slides are retrieved form the files more often than
not
the tape has separated from the slide with the tissue attached to the
tape.
I've repaired some of these using Sakura's suggested method of immersing
the
tape in xylene for a few minutes then reattaching to the slide.  This is
messy, time consuming and usually results in air bubbles.  Any
suggestions,
short of recutting all these slides?

Is there any way to prevent this from happening in the first place?
Right
now I'm ready toss the coverslipper out the window.

Thanks in advance,

Diane Coulter, RIH Histology 


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